Query Information

ORIGIN animal
BEGIN
>MDH
 MAHVCVVGAA GGVGQALSLL LTRSLPYGST LSLYDVVGAP GVAADLSHID
 NAGVTVKFAA GKIGVTRDPA LAALATGVDV FVFVAGGPIM PGMKRDDLFN
 STAGIVLDLV MTCASSSPKA MFCMISNPVN STVPIAAEVL KKLGVYNKNR
 LLGVTRLDML RATRFINEAR MPLVVDRVPV VGGHSDNSIV PLFHQLQGPL
 PPKEQLDKIT LRVQSAAYEV IDAKGGRGSA TLAMGEAGAR FVLDVVKGLT
 GASNPLVYAY VDTDGQSESE FLAIPVILGK SGIERRLPIG PMTESEKKLV
 DVAISIVKKN IEKGKEFALS KL

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MDH          322 Residues
Species classification: 4

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 4 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 11   Charge diffirence(C-N): -1.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   19
     Peak Value of UR:   2.24
     Net Charge of CR: 1
     Discriminant Score:      3.90
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -0.8
     Possible cleavage site: 24
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -3.77  threshold: -2.0
     INTEGRAL    Likelihood = -3.77   Transmembrane   70 -  86 (  68 -  87)
     PERIPHERAL  Likelihood = -1.75
     modified ALOM score:   0.85
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 69 (69 Residues)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 25
     TRSLPY
Discrimination of mitochondrial target seq.: 
     negative (-1.74)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 21%
     Larger value (>30%) is favared for ER memb. proteins
Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
SKL motif (signal for peroxisomal protein): 
     pos: 320(322), count: 1   SKL
SKL motif found at the C-terminus
     SKL score (peroxisome):  0.5
Amino Acid Composition Tendency for Peroxisome:  -2.77
Peroxisomal proteins?   Status: positive
Amino Acid Composition tendency for lysosomal proteins
     score:  0.74  Status: notclr
Checking the consensus for Golgi
Checking the consensus for Golgi
Checking the cytoplasmic tail of type II (Golgi)
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.811(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.640(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.500(Affirmative) < succ>
                       Golgi body --- Certainty= 0.370(Affirmative) < succ>


----- The End -----