OUTLINE
Experimental splenectomy in the mouse is used to
study
the role of the spleen in platelet destruction..
PROTOCOL
General anesthesia
- inject i.p. 250µl/mouse of the ANESTHETIC mixture
< wait for 2-10min for the anesthetic effect>
- for verify the anesthetic effect check plantar
reflexes (animal must be unresponsive to squeezing hind feet)
Preparation of the operation area
1. wash the surgical and adjacent area with C 2
H5OH-70%
2. raze the fur with the razor
3. wash the surgical area with C2
H 5
OH-70%
Operation
- At the left hypochondrium make a 1.5-2cm "skin,
muscles and
peritoneum" incision with surgical scissors
- Gently trap and push the spleen free of adjacent
tissue, separate
its ìlegî with blunt-ended scissors or forceps
- By a single knot tie off the artery with a 3-0 plane
CATGUT
by looping the suture through the mesentary (the artery enters the
spleen
at the superior polus). If necessary by a single knot tie off the
efferent
venule (exits the spleen at the inferior polus)
- Cut away the connective tissue and remove the spleen
- Apply on the open wound 1 pinch of Astrexene
(Chlorhexidin HCl) (5mg/g)
- Apply on the peritoneum and muscles the ìUî-like
suture with
plane CATGUT (3-0)
- Apply on the skin the ìUî-like suture with ETHICON
MERSILENE
(3-0)
Wakening
1. inject 250ul/mouse of the "WAKENING
MIXTURE"
Post operation period
1. after the operation took place let the animals to
rest for
at least 7 days in a cage with the filter
2. observe animals every day
SOLUTIONS
- ANESTHETIC MIXTURE=mix 1ml of Domitor (Medetomidin
HCl) (1mg/ml) , 1ml of Imalgene (Ketaminum HCl) (50mg/ml) and 8ml of
PBS <all steril>.
- "WAKENING MIXTURE"=mix 1ml of ANTISEDAN (Atipamezole
HCl) (5mg/ml) and 9ml of PBS <all sterile!>
ADDITIONAL INFO
Survey the mice
for some time after this operation.
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