::   L o w r y   a s s a y  ::

b a c k  ]
tube
BSA (0.5 mg/ml), l
H2O or NaCl, l
Protein, g
1
---
200
0
2
5
195
2.5
3
10
190
5
4
20
180
10
5
40
160
20
6
80
120
40
7
160
40
80

To each tube add 1 ml of Oxydative Solution:

50 ml Na2CO3 , 2% in NaOH 0.1 N + CuSO4, 1% 0.5 ml + NaK tartrate, 2% 0.5 ml
Mix and allow to stand for 10 min at 21C

Add 100l of FOLIN-CIOCALTEU's phenol reagent, 50% water solution
vortex immediately
Let to stay for 60 min at RT in dark

Read at the wave length of  740 nm
Draw the standard curve for a refference protein (BSA) (O.D/conc. of protein)
Calculate the concentration of the sample