l a b o r a t o r y   r e s e a r c h   i n   i m m u n o l o g y   ::   v i r o l o g y   ::   h e m a t o l o g y
virus
bar
v i r a l   i m m u n i t y   &   p a t h o g e n e s i s   g r o u p

::  p r o t o c o l  ::  b l e e d i n g  t i m e  ::


ƒ



OUTLINE

Although in the Review Article : "Idiopathic Thrombocytopenic Purpura: A practice Guideline Developed by Explicit Methods for the American Society of Hematology" bleeding time is considered as unnecessary/inapropriate to establish the diagnosis of ITP it's still used in experimental models of ITP study as an additional test.

PROTOCOL
  1. Anesthesize a mouse
  2. Make a standard incision on the dorsal tail vein using Simplate blades
  3. Immerse the tail in NaCl 0.9% at 37.5ƒC
  4. Record the time from the moment blood is observed to emmerge from the wound until cessation of blood flow
ADDITIONAL INFO
Alternatively you can perform a standard incision with an automatic device (Surgicutt; ITC, Edison, NJ) and every 15 secs apply a filter paper (Whatman 3MM) for 5 sec until the blood no longer stained it.
REFERENCES
Consequences of GATA-1 deficiency in megakaryocytes and platelets. Carl W; Jackson et al. Blood Vol.93 No9 May 1) 1999 : pp2867-2875
created by Andrei Musaji          ::          updated : 2004